Fig. 3.

TUNEL assay to detect intranuclear DNA fragmentation in apoptotic mNSC. (A) Visualization on day 7 and (B) quantification on days 0, 5 and 7 of TUNEL-positive cells (indicated with white arrows). In all microscopy images, the left-hand column represents data shown in the green (FITC) channel, the middle column represents data shown in the blue (DAPI) channel and the data shown in the right-hand column represents merged data. Scale bar = 100 μm. Data represent mean ± SEM, n = 8. * indicates significance p < .0001 compared to day 0. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)