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. 2018 Dec 4;9:2846. doi: 10.3389/fimmu.2018.02846

Table 2.

Twenty-nine splice events identified in 30 related rhesus macaques by PacBio sequencing of KIR transcripts.

Splice event Deletion/Inclusion Size (bp) Position Observed in KIR genes Result
Exon skipping (“cassette” exon) Deletion 300p Exon 4 3DL05, 3DL20, 3DS05 Missing D1 domain
Deletion 294 Exon 5 3DL01/02/05/07/10, 3DL10A/3DL02 Missing D2 domain
Deletion 51 Exon 6 2DL04 Missing stem region
Deletion 53 Exon 8 2DL04 Missing first region cytoplasmic tail
Deletion 594 Exons 4 + 5 3DL07, 3DL20 Missing D1 and D2 domain; possible KIR1D receptor
Deletion 446 Exons 5, 6 and 7 3DL01/05/07/10, 3DL10A/3DL02 Missin D2 domain, stem region, and transmembrane region
Deletion 155 Exons 6 and 7 2DL04 Missing stem and transmembrane region; possible soluble receptor
Alternative 3′ ss Deletion 36 Start exon 4 3DL01/02/05/07/08/10, 3DL20, 3DS01/02/03/05/w08, 3DL02/3DL08A In-frame; Missing 12 AA in the beginning of the D1 domain
Deletion 216 Start exon 4 3DL02/08, 3DS05/w08, 3DL02/3DL08A In-frame; Missing 72 AA in the beginning of the D1 domain
Deletion 267 Start exon 4 3DL05, 3DS05 In-frame; Missing 89 AA in the beginning of the D1 domain
Deletion 27 Start exon 5 2DL04 In-frame; Missing 9 AA in the beginning of the D2 domain
Deletion 115 Start exon 5 3DL07/08/10, 3DL20, 3DS03, 3DL02/3DL08A, 3DL10A/3DL02 Out-frame; Stopcodon introduced
Deletion 150 Start exon 5 3DL02/w03/10 In-frame; Missing 50 AA in the beginning of the D2 domain
Deletion 176 Start exon 5 3DL07, 3DL10A/3DL02 Out-frame; Stopcodon introduced
Inclusion 109 Following exon 7 2DL04 Out-frame; Stopcodon introduced
Inclusion 147 Following exon 7 2DL04 Stopcodon introduced
Inclusion 245/246 Following exon 7 2DL04, 3DL07 Stopcodon introduced
Alternative 5′ ss Deletion 198 End exon 3 3DL02, 3DS05, 3DL02/3DL08A In-frame; Missing 66 AA in the end of the D0 domain
Inclusion 109 Following exon 3 2DL04 Stopcodon introduced
Alternative 3′ and 5′ ss Deletion 81 Within exon 4 3DS02 In-frame; Missing 27 AA in center of the D1 domain
Deletion 107 Within exon 4 3DS02 Out-frame; Stopcodon introduced
Deletion 141 Within exon 4 3DL08, 3DSw08, 3DL02/3DL08A In-frame; Missing 47 AA in center of the D1 domain
Deletion 414 Parts exons 3 and 4 3DS05 In frame; Missing parts D0 and D1 domains
Deletion 324 Parts exons 4 and 5 3DL20 In frame; Missing parts D1 and D2 domains
Exkon skipping + Alt. 3′ ss Deletion 415 Exon 4 and start exon 5 3DL20 Out-frame; Missing the D1 domain and stopcodon introduced
Cryptic exon Inclusion 88 Following exon 5 3DL01 Out-frame; Stopcodon introduced
Inclusion 147 Following exon 5 2DL04 Stopcodon introduced
Inclusion 47 Following exon 6 3DL07 Out-frame; Stopcodon introduced
Intron retention Inclusion 98/99 Following exon 8 2DL04 “98 bp” alleles: out-frame; Stopcodon introduced “99 bp” alleles: in-frame; 33 additional AA′s subsequent to exon 8

The events are listed per alternative splicing mechanism. The size of deletions or inclusions are indicated in base pairs (bp). The result of the alternative splice event is predicted. The background color of each splice event corresponds to the background color of the different lineages illustrated in Figure 2B.