Table 3.

Three rhesus macaque samples were pooled for a PacBio Sequel sequencing run, which provided a four-fold coverage compared to the obtained transcriptome profiles.

Splice event		Deletion/Inclusion	Size (bp)	Position	Observed in KIR genes	Result
High coverage	Alt. 5′ ss	Deletion	209	End exon 4	3DL20	Out-frame; Stopcodon introduced
	Alt. 3′ ss + Alt. 5′ ss	Deletion	48	In exon 5	3DL05, 3DL08, 3DS02	In-frame; Missing 16 AA in the beginning of the D2 domain
	Exon skipping	Deletion	345	Exons 5 + 6	3DL20	Missing the D2 domain and stem region
Additional families	Alt. 3′ ss + Alt. 5′ ss	Deletion	97	In exon 4	3DL02, 3DSw08, 3DSw09	Out-frame; Stopcodon introduced
	Alt. 3′ ss	Deletion	79	Start exon 5	3DL10A/3DL08, 3DL11	Out-frame; Stopcodon introduced. 3DL11*009: ORF restored in cytoplasmic tail
	Alt. 3′ ss + Alt. 5′ ss	Deletion	112	Parts exons 4 + 5	3DL07, 3DL11	Out-frame; Stopcodon introduced
	Exon skipping	Deletion	645	Exons 4 + 5+ 6	3DL05, 3DL20	Missing D1 and D2 domains, and stem region

Three additional splice events were confirmed (≥10 PacBio reads, or confirmed in two macaques). Also, the alternative splicing profiles of three additional families, consisting of 25 rhesus macaques in total, were characterized. Only a single splice event was identified that was not observed in the main dataset, whereas three previously observed, but not confirmed, splicing events (italics) were confirmed by the additional rhesus macaque families.