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. 2018 Dec 4;9:2850. doi: 10.3389/fimmu.2018.02850

Figure 3.

Figure 3

IL-33 increases the secretion of IFN-γ by Gag and CEF stimulated CD4+ T cells. CD4+ T cells were isolated from HIV-1 individuals and treated with Gag peptide pools with rhIL-33 (0.1 ng/mL and 1 ng/mL) or without IL-33 (0 ng/mL). Intracellular IFN-γ expression was detected by flow cytometer and compared by paired t-test (0 ng/mL: 1.88 ± 1.06%; 0.1 ng/mL: 4.48 ± 1.98%; 1 ng/mL: 4.52 ± 1.73%). Representative flow cytometry dot plot (A) and summary data (B) were shown. CD4+ T cells were isolated from HIV-1 individuals and treated with CEF peptide pools with rhIL-33 (0.1 ng/mL and 1 ng/mL) or without IL-33 (0 ng/mL). Intracellular IFN-γ expression was detected by flow cytometer and compared by paired t-test (0 ng/mL: 1.64 ± 0.74%; 0.1 ng/mL: 2.60 ± 1.38%; 1 ng/mL: 4.06 ± 1.60%). Representative flow cytometry dot plot (C) and summary data (D) were shown.