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. 2018 Dec 4;9:2812. doi: 10.3389/fimmu.2018.02812

Figure 2.

Figure 2

H5N1 virus induces the agglutination and activation of γδ T cells. γδ T cells were infected with HPAI H5N1 viruses or H1N1 viruses at an MOI of 4 for 12 h. (A) Representative images of agglutination of γδ T cells induced by viruses. Allantoic fluid (AF) with no viruses as control. (B) Quantification of the agglutination of γδ T cells induced by viruses. (C) Flow cytometry analysis of the CD69 expression on γδ T cells following viral infection. (D) Quantification of CD69 expression levels on γδ T cells from at least three independent experiments. (E) ELISA analysis of the cytokine secretion by γδ T cells following viral infection. (F) Detection of viral M1 protein by Western blotting following viral infection. Data are presented as the means ± SEMs. *P < 0.05, **P < 0.01 and ***P < 0.001, Student's t-test.