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. 2018 Dec 4;9:2812. doi: 10.3389/fimmu.2018.02812

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Copyright © 2018 Dong, Ju, Yan, Zhang, Cai, Wang, Chen, Hu, Cui, Zhang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rH5HA proteins induce the agglutination and activation of γδ T cells. (A) Representative images of cultured γδ T cells which were seeded into 48-well plates and then treated with PBS, HAF or HA-his for 12 h. (B) Flow cytometry analysis of CD69 expression on γδ T cells following stimulation by HAF or HA-his proteins. (C) IFN-γ secretion by γδ T cells following stimulation by HAF or HA-his proteins. (D) Changes in the intracellular Ca²⁺ levels in γδ T cells following stimulation by HAF or HA-his proteins. Graphs in the panels represent the kinetics of the intracellular Ca²⁺ levels. Data are presented as the means ± SEMs. ^*P < 0.05, ^**P < 0.01, Student's t-test.