Table 1.
Polyunsaturated fatty acid containing phospholipid species and arachidonic acid to docosahexaenoic acid ratio in the cortex of repetitive mTBI hTau mice.
| Polyunsaturated fatty acid containing lipid species | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phosphatidylcholine (PC) | Phosphatidylethanolamine (PE) | Phosphatidylinositol (PI) | Lysophosphatidylcholine (LPC) | ||||||||||||||
| PUFA | AA to DHA ratio | PUFA | AA to DHA ratio | PUFA | AA to DHA ratio | PUFA | AA to DHA ratio | ||||||||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | ||
| 24H | 107.658 | 1.529 | 1.743 | 0.014 | 196.096 | 4.875 | 0.546 | 0.003 | 52.232 | 1.229 | 39.698 | 0.672 | 1.668 | 0.249 | 1.122 | 0.101 | |
| 3M | 105.477 | 3.827 | 1.770 | 0.016 | 183.554 | 9.720 | 0.571 | 0.003 | 48.719 | 2.025 | 27.743 | 2.879 | 2.206 | 0.152 | 1.204 | 0.029 | |
| SHAM | 6M | 113.526 | 9.569 | 1.746 | 0.015 | 187.340 | 6.213 | 0.554 | 0.004 | 48.254 | 0.437 | 33.317 | 1.720 | 1.936 | 0.263 | 1.208 | 0.022 |
| 9M | 110.043 | 2.299 | 1.770 | 0.017 | 210.230 | 7.798 | 0.554 | 0.004 | 58.315 | 3.229 | 35.581 | 1.955 | 1.819 | 0.234 | 1.229 | 0.029 | |
| 12M | 108.021 | 3.667 | 1.743 | 0.012 | 209.777 | 3.985 | 0.550 | 0.003 | 61.830 | 3.377 | 33.970 | 2.783 | 1.706 | 0.203 | 1.244 | 0.010 | |
| 24H | 120.880+++ | 1.800 | 1.758 | 0.009 | 216.893 | 3.416 | 0.565++ | 0.004 | 61.180++ | 1.476 | 39.486 | 0.672 | 1.664 | 0.179 | 1.222 | 0.025 | |
| 3M | 102.138 | 1.074 | 1.801 | 0.016 | 182.042 | 6.539 | 0.571 | 0.004 | 49.287 | 1.709 | 34.407+ | 1.342 | 1.764 | 0.182 | 1.268 | 0.021 | |
| r-mTBI | 6M | 106.410 | 2.022 | 1.769 | 0.011 | 178.112 | 17.649 | 0.569 | 0.004 | 50.289 | 0.939 | 35.988 | 2.177 | 2.079 | 0.251 | 1.270 | 0.021 |
| 9M | 104.953 | 4.297 | 1.781 | 0.010 | 188.896 | 8.696 | 0.565 | 0.003 | 50.972 | 4.222 | 35.590 | 1.500 | 1.645 | 0.244 | 1.246 | 0.029 | |
| 12M | 109.668 | 2.492 | 1.781 | 0.006 | 208.806 | 4.911 | 0.560 | 0.003 | 58.471 | 2.978 | 32.618 | 2.728 | 1.587 | 0.173 | 1.207 | 0.019 | |
Significant changes in polyunsaturated fatty acid containing PC, PE, PI, and LPC species, and arachidonic acid to docosahexaenoic acid ratio for the same species in the cortex of repetitive mTBI hTau mice. Sample size for all groups across all time points is n = 4. All data represent mean μM per (10 mg) wet weight ±SEM. Individual molecular lipid species were quantified by liquid chromatography/mass spectrometry and were summed after LipidomeDB analyses to generate polyunsaturated fatty acid classification for each phospholipid species, and a ratio of arachidonic acid to docosahexaenoic acid was compiled from these values. Asterisks represent +P < 0.05, ++P < 0.01, +++P < 0.001 for comparisons between sham/r-mTBI mice. PE, phosphatidylethanolamine; PC, phosphatidylcholine; PI, phosphatidylinositol; AA, arachidonic acid; DHA, docosahexaenoic acid.