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. 2018 Dec 4;9:1389. doi: 10.3389/fphar.2018.01389

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Copyright © 2018 She, Ma, Liu, Zhang, Qiu, Chen, Li, Xue, Luo, Wang, Xu, Zang, Zhao, Cao, Shen, Peng, Zhao, Yu, Chen, Nie, Shen, Chen, Chen, Qin, Dai, Chen and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of EESC is reversible. (A) ACh induced similar contractions in TRs after washout of EESC. (B) ACh-caused contractions were similar in TRs treated with EESC for 24 h and controls. NS: P > 0.05. These data suggest that EESC-induced relaxation is not due to EESC-induced cytotoxicity and inhibition of the contraction-related protein expression.