Table 2.

Technical validation of RNA-seq experiment by qPCR: list of validated genes, log₂ of fold change values (log₂FC) between high (H) and low (L) groups and Pearson correlations between expression values obtained from each technique.

Gene	log2FC RNA-seq	log2FC qPCR	Pearson correlation	p-Value
ADIPOQ	1.31	1.33	0.571	0.053
ATF3	-1.17	-1.22	0.984	6.92 × 10-9
CNOT10∗	-0.25	-0.14	0.642	0.024
DCAF5∗	-0.26	0.04	0.821	0.001
DNAJA1∗	-0.57	-0.53	0.961	1.01 × 10-8
EGR1	-1.64	-1.71	0.962	5.63 × 10-7
ELOVL6	1.50	0.83	0.807	0.002
FASN	2.54	1.19	0.808	0.001
FOS	-1.67	-1.65	0.961	6.72 × 10-7
GPHN∗	-0.05	-0.10	0.550	0.064
ITGB6∗	0.12	-0.18	0.758	0.004
PFKFB3	-1.10	-0.67	0.981	1.83 × 10-8
PLIN1	1.39	1.51	0.959	8.89 × 10-7
SCD	1.55	1.58	0.857	3.67 × 10-7
SPP1	-1.62	-1.57	1.000	6.67 × 10-18

^∗No differentially expressed in RNA-seq experiment; concordance correlation coefficient (CCC) value = 0.98.