Figure 1.

Treatment with Oncolytic Virus Controls the Growth of Both Injected and Non-injected Tumors

Hamsters were treated on days 1, 8, 15, 22, and 29 with 1 × 10⁸ VPs intratumorally (i.t.) and with 5 × 10⁷ TILs on day 1 intraperitoneally (i.p.). The growth of injected (A) and non-injected (B) hamster tumors (n = 5–6) was measured every 2–3 days until day 33. During the follow-up period, two animals were sacrificed from the mock group (day 24), two animals from the group receiving TILs only (day 22), and one animal from groups treated with Ad5/3-E2F-d24-hTNF-α (day 29) and Ad5/3-E2F-d24-hIL-2 (day 29). Small amounts of viral DNA were detectable also in non-injected tumors on day 16 (C). There were no differences between the injected and non-injected tumor sizes on day 33 (D). The graphs show mean plus SEM. Statistical differences were evaluated with mixed model analysis; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.