Figure 3.

Treatment with Armed Viruses Induces Positive Changes in Immune Cells in the Tumor Microenvironment

Tumor samples were collected on day 8, and the presence of natural killer cells (A), mature dendritic cells (B), macrophages (C), and M2-like macrophages (D) in tumors was detected with flow cytometry. Melanoma-specific T cells were detected with pentamers and the results with OVA⁺, Trp2⁺, and gp100⁺ cells pooled into one graph (E). Statistical differences were analyzed against corresponding mock tumor. The bars show mean plus SEM. Black bar indicates mock control, white bars unarmed virus or OT-I controls, and gray bars treatment groups with armed viruses. Unpaired t test was performed to analyze statistical differences; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.