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. 2018 Nov 29;103(6):874–892. doi: 10.1016/j.ajhg.2018.10.018

Figure 1.

Figure 1

Preliminary Validation of ATAC-seq Catalogs Generated from Ex Vivo DA Neurons

(A) The midbrain (MB) and forebrain (FB) of E15.5 brains from Tg(Th-EGFP)DJ76Gsat mice are microdissected, dissociated, and isolated by FACS.

(B) Read pileup and called peaks for the MB and FB libraries at the Th locus.

(C and D) Chromatin accessibility, genome-wide, is correlated between replicates.

(E) The sequences underlying MB and FB peaks display a high degree of evolutionary sequence constraint as measured by phastCons scores.

(F and G) For both the MB and FB, gene ontology terms of the expressed genes nearest to each peak reflect the neuronal origin and function of these catalogs.