FIG 1.

MxB inhibits HCV infection. (A) Jc1 HCVcc was used to infect Huh7.5.1 cells, which were treated with doxycycline (Dox) to express MxB. Levels of MxB protein and HCV core proteins were determined by Western blotting at 72 hpi. The band densities for core and actin were quantified by use of NIH ImageJ software. (B) Levels of HCV RNA in infected cells were quantified by qRT-PCR. The GAPDH mRNA level was also determined, and the data were used as an internal control to normalize the level of viral RNA. (C) HCV infection was determined by measuring Gluc activity in the supernatant. (D) Immunofluorescence staining of HCV core protein (green) and MxB (red). Nuclei were visualized by DAPI staining (blue). (E) Colocalization coefficients (Pearson and Manders coefficient values) of the HCV core protein (green) and MxB (red) shown in panel D were determined for randomly selected cells (>30) by use of Image-Pro Plus 7.0C software. Data are representative of at least three independent experiments, and values are expressed as means ± SD. For panels A to C, data are normalized to the control group, with the control value arbitrarily set to 1.