TABLE 1.
Summary of data collection, image-processing parameters, and refinement statistics
| Parameter | Value for: |
|
|---|---|---|
| AAV5-HL2476 complex | AAV5 alone | |
| Total number of micrographs | 254 | |
| Defocus range (µm) | 1.16-3.48 | |
| Electron dose (e−/Å2) | 75 | |
| Frames/micrograph | 50 | |
| Pixel size (Å/pixel) | 1.05 | |
| Starting no. of particles | 1,140 | |
| No. of particles used for final map | 1,026 | |
| B-factor used for final map (Å2) | −25 | |
| Resolution of final map (Å) | 3.11 | |
| PHENIX model refinement statistics | ||
| Residue range | 205-724 (AAV5) 21-234 (LC) 20-236 (HC) | 205-724 |
| Map CC | 0.706 | 0.885 |
| RMSD (bonds) (Å) | 0.01 | 0.01 |
| RMSD (angles) (Å) | 0.91 | 0.85 |
| All-atom clash score | 13.37 | 10.76 |
| Ramachandran plot | ||
| Favored (%) | 94.1 | 96.7 |
| Allowed (%) | 5.2 | 3.3 |
| Outliers (%) | 0.7 | 0 |
| Rotamer outliers (%) | 0.12 | 0 |
| C-β deviations | 0 | 0 |