TABLE 2.
Analysis and mutational outcome of the AAV5-HL2476 binding interface
| Residue VP3 | Location | HL2476 interaction residue | Distancea (Å) (interaction type) | AAV5 variant(s) | HL2476 escape |
|---|---|---|---|---|---|
| N443 VP3I | VR-IV | N51 (LC, CDR1) | 3.2 (HB) | N443Q, N443T | No |
| T444 VP3I | VR-IV | N73 (LC, CDR2) | 3.8 (HB) | T444V | No |
| R471 VP3A | VR-V | D74 (HC, CDR2) | 3.0 (HB) | R471E | No |
| V481 VP3A | VR-V | Y70 (LC, CDR2), Y122 (HC, CDR3) | 3.4 (VDW) | V481T, V481P, V481Y | No (V481T), partial (V481P), yes (V481Y) |
| R483 VP3A | VR-V | F52 (LC, CDR1), Y112 (LC, CDR3) | 3.8 (HB) | R483A, R483K, R483Q | Yes |
| A484 VP3A | VR-V | Y70 (LC, CDR2) | 3.4 (VDW) | ΔA484, A484S, A484Q | No (ΔA484, A484S), yes (A484Q) |
| T520 VP3A | VR-VI | R50 (HC, CDR1) | 2.5 (HB) | T520A, T520R | No |
| S576 VP3I | VR-VIII | P123 (HC, CDR3), Y70 (LC, CDR2) | 3.6 (HB) | S576A, S576Q | No (S576A), yes (S576Q) |
| T577 VP3I | VR-VIII | H111 (LC, CDR3) | 3.6 (HB) | T577A, T577V | No |
| T578 VP3I | VR-VIII | W52 (HC, CDR1) | 3.5 (HB) | T578A, T578Q | No |
a
HB, hydrogen bond; VDW, van der Waal contact.