FIG 16.

Intracellular trafficking of Alexa Fluor 488-labeled HAdV26 in A549, A549-B4, and A549-E6 cells. (A) Cells were incubated with Alexa Fluor 488-labeled HAdV26 (50,000 vp/cell) for 2 h at 37°C. Noninternalized viruses were rinsed away, and the cells were fixed with 2% paraformaldehyde (PFA). Green, Alexa Fluor 488-labeled HAdV26; blue, nuclei stained with DAPI; red, actin cytoskeleton stained with phalloidin. The images are maximum projections of confocal stacks. Representative confocal images are shown. Scale bars = 25 μm. (B) Quantification of virus internalization efficiency, expressed as virus number per cell. The horizontal bars represent means, and the error bars indicate standard deviations; the numbers of cells analyzed are indicated.