FIG 3.

(A and B) Binding and internalization of HAdV5, HAdV26, HAdV35, and HAdV26F35 in A549 cells (A) and in SK-OV-3 cells (B). The results are expressed as fold control value, i.e., the value obtained for HAdV5, plus standard deviations. (C) Binding and internalization of HAdV26 in A549 and SK-OV-3 cells. The results are presented relative to A549 plus standard deviations. For both binding and internalization, cells were first incubated with HAdV5, HAdV26, HAdV35, and HAdV26F35 on ice for 1 h, Multiplicity of infection (MOI), 1,000 viral particles (vp)/cell. To measure binding, unbound viruses were removed by rinsing the cells with cold trypsin and PBS and collected by scraping the cells. For internalization measurement, unbound viruses were removed as described above, and the cells were transferred to 37°C and incubated for 1 h, allowing the viruses to enter the cells. The Cells were then rinsed twice with warm trypsin, dispersed, and pelleted by centrifugation. For both binding and internalization, total (cellular plus viral) DNA was extracted from the cells and used for quantification of viral DNA by qPCR, using the CMV region as a target sequence. **, P < 0.01; ***, P < 0.001. Results are from three independent experiments (n = 3).