FIG 5.

Transduction efficiencies of HAdV5 (A), HAdV26 (B), HAdV35 (C), and HAdV26F35 (D) in A549 cells after downregulation of CAR, CD46, and/or αv integrin by specific siRNA transfection. Cells were transfected with specific siRNA at a final concentration of 50 nM and infected 48 h later with HAdV5, HAdV26, HAdV35, and HAdV26F35 at an MOI of 1,000 vp/cell. Transduction efficiency was measured by luciferase activity assay 48 h after infection. The results are presented as fold control, i.e., cells transfected with scrambled siRNA, plus standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Results are from three independent experiments (n = 3).