FIG 6.

Transduction efficiencies of HAdV5, HAdV26, HAdV35, and HAdV26F35 in SK-OV-3 cells after downregulation of CD46 and/or αv integrin by specific siRNA transfection. Cells were transfected with specific siRNA at a final concentration of 50 nM and infected 48 h later with HAdV5, HAdV26, HAdV35, and HAdV26F35 at an MOI of 1,000 vp/cell. Transduction efficiency was measured by luciferase activity assay 48 h after infection. The results are presented as fold control, i.e., cells transfected with scrambled siRNA, plus standard deviations. *, P < 0.05; **, P < 0.01. Results are from three independent experiments (n = 3).