Transduction efficiencies of HAdV5, HAdV26, HAdV35, and HAdV26F35 in M21L and M21L4 cells. Cells were infected with HAdV5, HAdV26, HAdV35, and HAdV26F35 at an MOI of 1,000 vp/cell. Transduction efficiency was measured by luciferase activity assay 48 h after infection. M21L cells are αv integrin negative, and M21L4 cells are αv integrin positive. The results are presented as absolute values in RLU per milligram of protein and are shown as means plus standard deviations. ***, P < 0.001. Results are from two independent experiments (n = 2).