FIG 3.

NF449, A438079, and AZT block HIV-1 replication in HLACs. HLACs were collected at 0, 2, 5, 8, and 12 dpi. (A) HLACs were analyzed by flow cytometry to quantify viable cells. (B) HLACs were analyzed by flow cytometry for productive infection by NL-CI mCherry fluorescence. (C) NF449, A438079, and AZT were tested for dose-dependent inhibition in infected HLACs at 8 dpi by a 1:5 titration down from 100 μM. Open circles indicate viability, and filled circles indicate percent inhibition of productive infection. Mean values ± standard errors of the means are presented from three donors. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.