FIG 4.

HIV-1 infection and inhibition in human tonsil explant tissue blocks. (A) Human tonsil explant tissue blocks were collected, dissected, and plated in blocks on GelFoam for 24 h prior to infection. Blocks were infected with HIV-1 NL-CI. Supernatants were collected at 2, 5, 8, and 12 dpi. (Printed with permission from Mount Sinai Health System.) (B) Supernatants collected from human tonsil explant tissue blocks at the indicated times were measured for HIV-1 p24 by ELISA. Unfilled circles indicate uninfected samples, and filled circles indicate infected samples. (C) Supernatants from human tonsil explant tissue blocks on each day were tested for HIV-1 infectivity as quantified by TZM-bl assay. (D) Human tonsil explants were infected with HIV-1 NL-CI in the presence or absence of the indicated inhibitors (100 μM). Supernatants were collected at 2, 5, 8, and 12 dpi after infection. HIV-1 p24 antigen levels were measured by ELISA in samples in which infected tonsils were incubated in the presence or absence of the inhibitors NF449, A438079, and AZT at 100 μM. Data represent cumulative HIV-1 p24 production by adding the measurements at each successive time point. Mean values ± standard errors of the means are presented from six donors. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P < 0.0001.