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. 2018 Dec 4;9:2851. doi: 10.3389/fimmu.2018.02851

Figure 3.

Figure 3

Only fibroin/gelatin MPs induce expression of proinflammatory cytokines in MEF and BMDM, while both fibroin/gelatin and spidroin MPs downregulate profibrotic growth factors expression in MEF. (A) MEF monolayer after 1 week in culture on fibroin-gelatin (F/G) or spidroin (Sp) microparticles. The actin cytoskeleton is stained by phalloidin-Alexa488 (green), the nuclei are stained by Hoechst 33342. Scale bar−50 μm. (B) Adhesion efficiency of MEF and BMDM toward fibroin/gelatin and spidroin MPs (n = 4). (C,D) Expression of TNF (C) and IL-6 (D) by three cell types cultured on fibroin/gelatin or spidroin MPs, when compared with conventional 2D cultures (control) as revealed by qPCR after 6 h (left) and by ELISA in supernatants after 24 h (right). (E) Expression of fibroblast growth factors genes, Ctgf and Fgf2, by MEF, cultured for 6 h on fibroin/gelatin or spidroin MPs, when compared with soluble fibroin or spidroin (sF or sSp) and conventional 2D cultures (control) as revealed by qPCR analysis (n = 3). Data are representative of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.