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. 2018 Dec 4;9:2851. doi: 10.3389/fimmu.2018.02851

Figure 4.

Figure 4

Subcutaneous injection of fibroin/gelatin and spidroin MP affects infiltration of myeloid cells and reduces expression of profibrotic growth factors. (A) Representative dot plots, showing expression of Ly6C and Ly6G by myeloid cells isolated from the skin 1 day after injection with PBS, fibroin/gelatin or spidroin MPs (gating strategy—Supplementary Figure 2). (B) Total number of CD11b+ myeloid cells in skin samples. (C) Proportion of Ly6Chi and Ly6C− myeloid cells in skin samples. (D) MFI of Ly6C staining among Ly6G−Ly6Chi population of myeloid cells. All cells are VD−CD45+CD11b+. (E) Immunofluorescent staining of fibroin/gelatin and spidroin MPs with anti Ly6C-antibody 24 h after subcutaneous injection. Cell nuclei were stained with Hoechst 33342. (F–H) Expression of proinflammatory cytokines (F), chemokines (G), and profibrotic growth factors (H) in skin 1 day after injection with PBS, fibroin/gelatin or spidroin MPs. Data are representative of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.