Subject area	Biology
More specific subject area	Toxicology
Type of data	Table
How data was acquired	Yeast two-hybrid assay. Luminescence was read on a 96-well plate luminometer (Luminescencer JNR AB2100; Atto Corp., Tokyo, Japan)
Data format	Raw data
Experimental factors	583 chemicals of receptor binding activities, yeast toxicities, and photobacterium toxicities were tested. Each positive control was 17β-estradiol (hER-, medER-agonistic, β-naphthoflavone (AhR-agonistic)), 4-hydroxy-tamoxygen (ER-antagonistic), and 1-nitropyrene (AR-antagonistic).
Experimental features	The hERα-, medERα-, and AhR-agonist activities and hERα- and AR-antagonist activities of the 583 test chemicals were measured using a yeast two-hybrid assay system. The hERα, medERα, or human AhR and the coactivator TIF2 were introduced into each yeast cell (Saccharomyces cerevisiae strain Y190) in accordance with the method by Nishikawa [1].
Data source location	Tsukuba, Ibaraki, Japan
Data accessibility	All data are presented in this article.
Related research article	Kamata R., Shiraishi F., Nishikawa J., Yonemoto J. and Shiraishi H., 2008. Screening and detection of the in vitro agonistic activity of xenobiotics on the retinoic acid receptor. Toxicol. in vitro. 22, 1050–1061 [2].
	Kamata R., Nakajima D., and Shiraishi F., 2018. Agonistic effects of diverse xenobiotics on the constitutive androstane receptor as detected in a recombinant yeast-cell assay. Toxicol. in vitro. 46, 335–349 [3].