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. 2018 Dec 4;9:2817. doi: 10.3389/fimmu.2018.02817

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Copyright © 2018 Rohani, Dimitrova, Beppu, Wang, Jefferies and Parks.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tolerance to IMQ is dependent on MMP10. (A) Schematic diagram of in vivo tolerization protocol. (B,D) Back skin from wildtype and Mmp10^−/− mice treated with 1- or 2-hit model was homogenized and total RNA was isolated. Expression of target mRNAs was measured using qPCR and normalized to untreated (control) wildtype mice, n = 7–10 mice per group from two independent experiments. (C) Single cells isolated from back skin tissue were stained for CD45 and Ly6G. The frequency of Ly6G⁺ cells (a marker of neutrophils) on pre-gated CD45⁺ cells was assessed by flow cytometry. (p ≤ 0.05: ^*WT-Q vs. WT-QQ and Mmp10^−/−-Q vs. Mmp10^−/−-QQ;WT-Q vs Mmp10^−/−-Q and WT-QQ vs. Mmp10^−/−-QQ; ^**p ≤ 0.01, ^***p ≤ 0.001,p ≤ 0.01).