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. 2018 Dec 4;9:1668. doi: 10.3389/fpls.2018.01668

FIGURE 4.

FIGURE 4

Conservation of the ExxER/K motif in A. thaliana NPFs. Multiple sequence alignment of the TMH1 region of 52 Arabidopsis thaliana NPFs. Included in the sequence are two glycine residues located N-terminal to the TMH1, but not part of the α-helix. The ExxER/K motif (red box) is fully conserved in 31 AtNPFs (60%) (blue box), while ten AtNPFs (19%) completely lack the motif (green box). A logo was created to represent the motif sequence conservation. Amino acid numbering is based on the sequence of AtNPF6.3. Amino acids are colored based on their chemical properties (hydrophobic amino acids are black, polar are green, basic are red, and acidic are blue). The overall height of stacks represents the sequence conservation while the height of letters indicates the relative frequency of each amino acid in that position (Crooks et al., 2004).