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. 2018 Dec 10;6(23):e13939. doi: 10.14814/phy2.13939

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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mRNA (A) and protein levels of APJ receptor by Western Blot (B) in whole kidney extracts of db/m and db/db mice. APJ receptor protein (42 kD) was normalized to the mouse β‐tubulin (55 kD) (B). APJ mRNA and protein levels are significantly decreased in db/db mice. The bar graphs represent mean±SEM values; *, P < 0.05 versus db/m mice. Immunohistochemistry (C) of kidney sections from db/m (left) and db/db (right). APJ staining (dark brown) is seen in tubules and to lesser extent in glomeruli. Staining was stronger in db/m as compared to db/db mice.