Table 1.
Characteristics of db/m and db/db mice at 24–32 weeks of age
| db/m | db/db | |
|---|---|---|
| Blood glucose (mmol/L) | 163 ± 9 | 530 ± 41 |
| Body weight (g) | 27.8 ± 0.5 | 49.5 ± 2.0* |
| Kidney weight (mg) | 154 ± 9 | 206 ± 6* |
| Ratio kidneys/body weight (mg/g) | 5.6 ± 0.4 | 4.2 ± 0.2* |
| Albumin/Creatinine ratio (μg/mg) | 7.3 ± 2.0 (5) | 613.3 ± 366.0* (5) |
| Mesangial matrix expansion | 0.60 ± 0.24 (5) | 2.20 ± 0.20* (5) |
| Glomerular cellularity | 0.6 ± 0.24 (5) | 1.8 ± 0.20* (5) |
| Glomerular tuft area (μm2) | 2804.2± 111 (5) | 3931.5 ± 252.6* (5) |
| Podocyte count (per glomerulus) | 13.1 ± 0.3 (8) | 10.0 ± 0.4* (8) |
db/m control mice; db/db diabetic mice (n = 7 and n = 6 respectively, unless stated otherwise); Data are expressed as means ± SE.
*
P < 0.05 db/m versus db/db mice by Mann–Whitney test; Optical histology: mesangial matrix expansion and glomerular cellularity, were evaluated on H&E and PAS sections by a renal pathologist. Podocyte count was performed by counting WT‐1 stained nuclei in a blinded fashion by two independent investigators.