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. 2018 Dec 4;9:2913. doi: 10.3389/fmicb.2018.02913

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Copyright © 2018 Ünal, Berges, Smit, Schiene-Fischer, Priebe, Strowig, Jahn and Steinert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CdPrsA2 influences germination efficiency of C. difficile spores. Germination of C. difficile 630Δerm (wild type) and its isogenic ΔprsA2::ClosTron mutant (ΔprsA2) in the presence of (A) 1 mM deoxycholic acid (DCA) or (B) 5 mM taurocholic acid (TCA) was assessed in 96-wells by measuring the change in optical density of the spore suspension at OD₆₀₀ _nm over 20 min. Plotted is the change in optical density relative to the starting OD at t₀. Shown are the means and SEM of three measurements performed with spores of two different preparations. Statistical significance was calculated using Student’s t-test (^∗p ≤ 0.05).