Table 1.
Bacterial strains and plasmids used in this study.
| Name | Features | Reference |
|---|---|---|
| C. difficile str. 630Δerm | Erythromycin-sensitive derivative of strain 630 | DSMZ† (DSM 28645), (Hussain et al., 2005) |
| C. difficile str. 630ΔermΔprsA2 | cd35000::ClosTron | This study |
| E. coli DH10β | Strain for cloning and plasmid propagation, F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL λ- | Grant et al., 1990 |
| B. megaterium MS941 | Strain for recombinant production, deficient of the major secreted protease (ΔnrpM) | Wittchen and Meinhardt, 1995 |
| pSPYocH-hp | Vector for recombinant production in B. megaterium, PxylA-(-35+ rbs+)-spyocH-mcs-his6, TcR, AmpR | Stammen et al., 2010 |
†German Collection of Microorganisms and Cell Cultures.