Table 2.
Primers used in this study.
| Name | Sequence† | Features | Reference |
|---|---|---|---|
| PrsA2(wt)_For | TATactagtAGTAAAGGAGAAACTGTGGC | SpeI | This study |
| PrsA2(wt)_Rev | TATcggccgAGATCCACGAGGTACTAAGATTGTGATTTTATTTAT | EagI, Thrombin site | This study |
| PrsA2(K188A)_For | GCAACAGTAGATGATAATAACAAGCC | This study | |
| PrsA2(K188A)_Rev | CAATAAAATATGAGAAGCTTCTAC | This study | |
| PrsA2(D232A)_For | ATATTCACAAGCTACTTCAGCAAG | This study | |
| PrsA2(D232A)_Rev | TTTTTTGCTACTTTTGCAAAATCCTC | This study | |
| PrsA2(L241A)_For | GCAGGATTCTTTTCAAGGGGTCAA | This study | |
| PrsA2(L241A)_Rev | TTTACCACCATCACTTGCTGA | This study | |
| PrsA2(M249A)_For | GGTCAAGCGGTTGCTGAATTTG | This study | |
| PrsA2(M249A)_Rev | CCTTGAAAAGAATCCTAATTTACC | This study | |
| PrsA2(F253A)_For | GCAGAAGATGCTGCTTTTTCTATGAA | This study | |
| PrsA2(F253A)_Rev | TTCAGCAACCATTTGACCCC | This study | |
| PrsA2(T271A)_For | GCTCAATATGGATACCACATAATTAAAG | This study | |
| PrsA2(T271A)_Rev | TTCAACTAAATCAGATACTTCACC | This study | |
| PrsA2(Y273A)_For | GAAACTCAAGCTGGATACCACAT | This study | |
| PrsA2(Y273A)_Rev | AACTAAATCAGATACTTCACCCTTT | This study | |
| PrsA2(H276A)_For | TGGATACGCTATAATTAAAGTGACAG | This study | |
| PrsA2(H276A)_Rev | TATTGAGTTTCAACTAAATCAGATAC | This study | |
| EBS universal primer | CGAAATTAGAAACTTGCGTTCAGTAAAC | Heap et al., 2007 | |
| ErmRAM_F | ACGCGTTATATTGATAAAAATAATAGTGGG | Heap et al., 2007 | |
| ErmRAM_F | ACGCGTGCGACTCATAGAATTATTTCCTCCCG | Heap et al., 2007 | |
†Highlighted are the bases for the respective alanine exchange, restriction sites are kept in lowercase.