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Relative catalytic activity of alanine substitution mutants of CdPrsA2.
| PrsA2 | kcat/Km (M-1 s-1) |
|---|---|
| Wild type | (6.78 ± 0.41) × 104 |
| D232A | (1.80 ± 0.05) × 105 |
| M249A | (5.49 ± 0.16) × 104 |
| Y273A | (1.06 ± 0.24) × 103 |
| H276A | (2.61 ± 0.06) × 104 |
| K188A | (3.65 ± 0.40) × 104 |
| T271A | (1.06 ± 0.10) × 103 |
| F253A | (1.61 ± 0.23) × 103 |
| L241A | (5.02 ± 0.50) × 103 |
