Table 5.

Substrate specificity of wild type CdPrsA2 protein.

Xaaa	kcat/Km (M-1 s-1)
Ala	(3.21 ± 0.27) × 105
Leu	(8.50 ± 0.67) × 105
Ile	(4.26 ± 0.51) × 104
Val	(2.70 ± 0.23) × 104
Lys	(5.67 ± 1.30) × 105
Arg	(7.58 ± 1.85) × 105
Glu	(3.20 ± 0.26) × 104
Gln	(3.96 ± 0.08) × 105
Met	(5.77 ± 1.22) × 105
Phe	(3.33 ± 0.58) × 105
Tyr	(1.63 ± 0.32) × 105
Trp	(2.58 ± 0.40) × 105

^aAmino acids used to replace the position preceding proline in the substrate peptide Abz-Ala-Xaa-Pro-Phe-pNA.