Table 1. Subcellular localization analysis.
ICQ, Intensity correlation quotient; PSC, Pearson’s correlation coefficient. For ICQ, 0 stands for no colocalization and 0.5 stands for high colocalization; for PSC, 1 is total positive correlation and 0 is no correlation (Li et al., 2004; Reguera et al., 2015). Values are means ± se; 14 to 16 fluorescence images taken from seven different seedlings were collected for each statistical analysis.
Seedling | SYP32-YFP | GFP-SYP43 | GFP-VAMP727 | |||
---|---|---|---|---|---|---|
PSC | ICQ | PSC | ICQ | PSC | ICQ | |
KEA4-RFP | 0.76 ± 0.06 | 0.28 ± 0.01 | 0.47 ± 0.03 | 0.25 ± 0.02 | 0.43 ± 0.02 | 0.23 ± 0.01 |
KEA5-RFP | 0.61 ± 0.02 | 0.28 ± 0.02 | 0.58 ± 0.03 | 0.26 ± 0.01 | 0.29 ± 0.03 | 0.17 ± 0.01 |
KEA6-RFP | 0.71 ± 0.02 | 0.33 ± 0.01 | 0.41 ± 0.04 | 0.22 ± 0.01 | 0.35 ± 0.02 | 0.18 ± 0.01 |