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. 2018 Oct 17;178(4):1704–1719. doi: 10.1104/pp.18.00452

Figure 6.

Figure 6.

The 22°C to 29°C temperature increase reduces bri1-301 stability. A, Immunoblot analysis of BRI1/bri1-301 protein abundance. B, Immunoblot analysis of bri1-301 protein stability after the CHX treatment. C, Immunoblot analysis of BRI1/bri1-301 protein abundance in 18-d-old soil-grown seedlings (shown below the gel strips) of the wild type (WT), bri1-301, a pBRI1::bri1-301 bri1-701 line, and an F1 plant of the bri1-301 × bri1-701 cross. D, Photographs of 18-d-old seedlings grown at different temperatures. E, Immunoblot analysis of BRI1/bri1-301 protein abundance in the seedlings shown in D. F, Endo-H analysis of BRI1/bri1-301 proteins with total proteins extracted from wild-type and bri1-301 seedlings grown in 22°C and 29°C growth chambers. G, Photographs (top) and immunoblot analysis (bottom) of 20-d-old seedlings grown in soil at 29°C. H, Immunoblot analysis of trypsin sensitivity of the bri1-301 protein from the 22°C- and 29°C-grown bri1-301 mutant. TR, trypsin. In A to C, E, and H, the numbers shown in the space between two gel strips are BRI1/bri1 abundance (relative to that of the wild-type BRI1 in lane 1 in A and C, to that of nontreated samples in B and H, or to that of 18°C-grown seedlings of the same genotype in E) after normalization with the signals of corresponding ACTIN (A–C and E) or RbcS (H) bands. Bars in C, D, and G = 1 cm.