Supplementary Table 2. Preparation of ddPCR reaction mixture.

Component	Final concentration
ddPCR Supermix for Probes (no dUTP)	1 ×
Template DNA 1	–
Additional dNTP mixture	0.91 mM
Primers (forward and reverse primer)	0.45 µM each
Probes (input as a pair of WT and each mutant probe)
KRAS WT	0.45 µM
KRAS G12C	0.77 µM
KRAS G12D	0.45 µM
KRAS G12V	0.45 µM
KRAS G13 D	0.05 µM
KRAS G12A	0.68 µM
KRAS G12 R	0.07 µM
KRAS G12S	0.68 µM
KRAS G13C	0.68 µM
GNAS WT	0.45 µM
GNAS R201C	0.34 µM
GNAS R201H	0.45 µM

Total, 22 µL reaction volume.

¹1 – 4 µL of purified DNA were utilized.