Table 1. The amino acid (aa) and nucleotide states at the sites of the functionally relevant lysines before the Csd/Fem duplication and divergence event (Csd/Fem MRCA) and before the Csd allele divergence (Csd MRCA).
| aa/codon of Csd B2–25 | aa/codon of Csd/Fem MRCA (P > 0.9) | aa/codon of Csd MRCA (P > 0.9) | aa/codon polymorphisms (frequency %)3) | ||
|---|---|---|---|---|---|
| NLS | C1 | K14 | R | K | K (100%) |
| AAA | AGA | AAA | AAA | ||
| K16 | R | K | K (100%) | ||
| AAA | AGA | AAA | AAA | ||
| K24 | E | E | E (57%) K (43%) | ||
| AAA | GAA | GAA | GAA AAA | ||
| C2 | K243 | Q | K | K (100%) | |
| AAA | CAA | AAA | AAA | ||
| K248 | E | K | E (7%) K (93%) | ||
| AAA | GAA | AAA | GAA AAA | ||
| C3 | K259 | R | K | E (14%) K (79%) N (7%) GAG AAG AAC | |
| AAG | AGG | AAG2) | |||
| K280 | E 1) | K | K (21%) K (79%) | ||
| AAG | GAA | AAA | AAG AAA | ||
1)
Ambiguous codon (P < 0.9) due to indels that occurred with outgroup sequence comparison.
2)
The predicted codon was R (AGG), P < 0.6 using ANC-GENE (Zhang and Nei 1997), and K (AAG), P > 0.9 using MEGA (Tamura et al. 2011). From the more parsimonious number of mutations required to produce the other polymorphism (GAG and AAC), we suggest that the aa/codon of the csd MRCA is K/AAG.
3)
Estimated from a random sample of 14 csd alleles.