Figure 7.

Change in Xrn1 interactions in rad53∆ cells. A. Co-IP of Xrn1-Flag and Lsm3-GFP in wild type or rad53∆ cells. Asynchronous cells were untreated (-) or treated (+) with 0.05% MMS for 3 hr. Samples were immunoprecipitated with Flag antibodies. B. List of proteins identified in reciprocal SILAC mass spectrometry analyses of Xrn1-Flag IP from wild type and rad53∆ cells treated with 2 μg/mL 4-NQO for 60 min. C. Co-IP of Xrn1-Flag and Mss116-GFP or YMR196W-GFP in wild type or rad53∆ cells, as indicated. Asynchronous cells were untreated (-) or treated (+) with 0.05% MMS for 3 hr, and samples were immunoprecipitated with Flag antibodies.