Table 2.

Species and total reads used to create the simulated multi-species metagenome. It shows the size of the original database, in reads and bases, the reads and bases used to create the metagenome, the size of the reported genome, and the depth calculated as the bases used divided by the genome size

Species	NCBI reference	Phylum/Class	Total reads	Total bases (Mbp)	Used reads	Used bases (Mpb)	Genome size (Mpb)	Depth(x)
Synechocystis	DRR106442	Cyanobacteria/Cyanobacteria	589,689	21.9	112,805	41.5	3.5	11.7
Dokdonella	SRR4217676	Proteobacteria/Gammaproteo-bacteria	376,022	80.5	376,022	80.5	4.6	17.41
Hymnobacter	SRR1334914	Bacteroidetes/ Cytophagia	2,917,298	958.5	37,599	12.3	5.0	2.4
Microbacteriaceae	SRR5493999	Actinobacteria/Actinobacteria	1,815,433	382.4	37,599	7.9	3.2	2.4
Rhizobium	SRR5165471	Proteobacteria/Alphaproteo-bacteria	1,152,754	242.2	37,599	7.9	4.5	1.7