Fig. 1.

ABCG2 and MDR1 protein expression is correlated with resistance to cisplatin and paclitaxel in ovarian cancer cells. (A1 and A2) Comparison of ovarian cancer cell viability following CDDP and PAC treatment for 48 h. a and b) The indicated concentrations of CDDP and PAC were treated to three ovarian cancer cell lines for 48 h. Cell viability was determined by MTT assay. b ABCG2 and MDR1 protein expression was measured by Western blot. c-d. Protein expression levels were quantitated by densitometry and normalized to GAPDH. The ratio of densities was calculated using PA-1 as control. The correlation between CDDP IC50 and ABCG2/MDR1 protein expression, PAC IC50 and ABCG2/MDR1 protein expression were determined by simple linear regression analysis. The correlation coefficient square (R²) was determined by Pearson’s correlation coefficient test