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. 2018 Dec 10;18:1237. doi: 10.1186/s12885-018-5090-2

Fig. 3.

Fig. 3

PCV2 ORF3 overexpression induces nucleus fragmentation. B16F10 cells were transfected with either pcDNA3.1 empty plasmid (a and c) or pcDNA-ORF3 plasmid (b and d) and fixed 48 h post-transfection. Endogenous tubulin was stained with a FITC-labeled anti-α-tubulin antibody (green) in panels a and b. DNA was stained with DAPI (blue). Scale bars 5μm