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. 2018 Dec 11;8:64. doi: 10.1186/s13578-018-0262-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PolySia and cell migration. a HUVEC migration was examined by scratch assay in the absence (endoN treatment) or presence of polySia (no endoN). b Areas of untreated cell settings (control) were determined and set to 100%. Values are means of 18 scratches. The statistical evaluation was performed by paired Student’s t test (two tailed). Significance level is indicated by **p < 0.005. c The presence of polySia in the applied cell culture medium was assessed by immunostaining with anti-polySia antibody by Western blotting. Samples were pretreated with buffer (−) or endoN (+) before analysis