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Prortein detection for evaluation of E-selectin expression performed using western blotting technique. (A) Protein expression levels of E-selectin in HUVECs cells treated by thyroid-stimulating hormone (TSH) (1 and 2 μM) for 6 and 24 hand (B) TSH treatment significantly increased relative expression of E-selectin. For analysis protein's bands densities, Image J software was used to analyze the densities. Each protein (in one repeat) bands normalized to β-actin bands.
