Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 4;9:2864. doi: 10.3389/fimmu.2018.02864

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Xia, Qian, Xun, Hamon, Sartre, Formisano, Mailfert, Phelipot, Billaudeau, Jaeger, Nunès, Guo and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Naive CD4⁺ T cells show various calcium response modes upon different stimulation. Naive 3A9 TCRtg CD4⁺ T cells were loaded with Fluo-4 PBX before incubation at 37°C for 45 min with COS APC cells pulsed without or with different doses of HEL 48–63 as indicated. Time-lapse movies of T cells were made by confocal microscope as described in Materials and Methods. For each antigenic peptide concentration, modes of calcium response are represented as a pie chart: non-activated in gray, maintained in red, oscillatory in green, and unique in yellow. The total number of cells analyzed is indicated in parentheses. The percentage of activated (reacting) cells is shown by the double arrows, and their values are indicated.