Figure 1.

Quantification of circulating CD4⁺IFN-γ⁺, CD4⁺IL-17A⁺, and IL-22⁺ cells in each group. (A–C) Representative four-color dot plot analyses of CD4⁺IFN-γ⁺, CD4⁺IL-17A⁺, and IL-22⁺ cells after stimulation with PMA and ionomycin. (D) Frequency of CD4⁺IFN-γ⁺, CD4⁺IL-17A⁺, and IL-22⁺ cells on the gated lymphocytes in the FSC/SSC plot in each group. Results are represented as the mean ±SD (** p<0.05). ANOVA followed by Tukey’s post hoc test was used to analyze statistical significance.