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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Integr Biol (Camb). 2016 Aug 3;8(9):936–945. doi: 10.1039/c6ib00079g

Figure 6. Effect of CAT, CAC, CAG or CAA in the E-box motif (CAN∣NTG) on binding of TCF4-GST to 5hmC and Cytosine.

Figure 6.

A. E-box half site CAT∣NTG motifs binding DNA containing cytosine or 5hmC. ACAT∣GTG is only well bound with 5hmC, while CAT∣CTG is only bound with cytosine. B. E-box half site CAC∣NTG motifs binding DNA containing cytosine or 5hmC. Similar to CAT∣NTG, E-box motifs containing ACAC∣GTG preferentially bind DNA containing 5hmC, while CAC∣CTG preferentially binds DNA containing cytosine. C. E-box half site CAG∣NTG motifs binding DNA containing cytosine or 5hmC produces a more complex pattern. D. E-box motifs containing CAA∣NTG with cytosine or 5hmC are not well bound by TCF4-GST. DNA 8-mers containing E-box are red spots, 8-mers with a cytosine are black.

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