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. 2018 Dec 4;9:1432. doi: 10.3389/fphar.2018.01432

FIGURE 3.

FIGURE 3

PSTPIP2 silencing promotes FLSs inflammatory response. (A) The protein level of PSTPIP2 was analyzed by Western blot in FLSs with PSTPIP2 silencing. (B) The mRNA level of PSTPIP2 was analyzed by qRT-PCR in FLSs with PSTPIP2 silencing. (C) The mRNA levels of TNF-α, IL-1β, and IL-6 were analyzed by qRT-PCR in FLSs with PSTPIP2 silencing. (D) The protein levels of TNF-α, IL-1β, and IL-6 were analyzed by Western blot in FLSs with PSTPIP2 silencing. (E) The levels of TNF-α, IL-1β, and IL-6 in FLSs supernatants were detected by ELISA. The bands or images in the figure are representative in three independent experiments. Each group contains 5 rats. Data shown are the mean ± SD from three independent experiments. #P < 0.05, ##P < 0.01 versus NC-RNAi group; ∗P < 0.05, ∗∗P < 0.01 versus normal group.