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. 2018 Nov 15;115(11):2081–2086. doi: 10.1016/j.bpj.2018.10.022

Table 1.

Binding Stoichiometry at the Four ITC Peaks

Protein No. Transition Peak [SDS]aq (mM)a No. Bound SDSa
EXG:CBM 1 1.4
2 2.1 ± 0.2 14.6 ± 2.0
3 3.0 ± 0.1 28.4 ± 1.5
4 4.04 ± 0.02 42.1 ± 0.2
EXG:CBMQQQW 1 1.1
2 2.4 ± 0.2 12.0 ± 2.5
3 3.0 ± 0.2 25.6 ± 2.0
4 4.0 ± 0.2 42.8 ± 2.4
a

Parameters from fitting to Eq. 1 (in the Supporting Materials and Methods).