Spatially resolved tail order parameters of the sn-1 (top) and sn-2 (bottom) alkyl chains of phospholipids near a single cholesterol molecule. Dashed lines show the average tail order parameter for bilayers containing, 0, 4, and 6% cholesterol for comparison. Phospholipids in close proximity to a single sterol show slightly increased ordering, comparable to those in a bilayer with 4% cholesterol. To see this figure in color, go online.